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Intact Genomics

intact genomics

Intact Genomics offers high quality competent cells, enzymes, cloning kits, RT-PCR kits, and RT-qPCR kits at very competitive prices. ig™ competent cells have the highest transformation efficiencies in the market.  ig™ Taq DNA Polymerase, T4 DNA Ligase, Pfu DNA Polymerase, RT-PCR Kit, SYBR Green qPCR 2x Master Mix, and igScript™ First Strand cDNA synthesis kit have been tested and proven to be very high quality with robust and reliable results as well. Intact Genomics also provides a variety of custom services including High Molecular Weight (HMW) DNA preparation, BAC library, fosmid library, DNA library screening, custom vector construction, large-insert DNA cloning, and next generation sequencing. All our products and services are developed and manufactured from our state-of-the-art laboratories in St. Louis, USA.

www.intactgenomics.com

More information? Contact Us by e-mail info@divbio.pl

ElectroCompetent Agrobacterium Combo Pack 1290-24

Agro-combo


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Specyfikacja

50μL
Intact Genomics ElectroCompetent Agrobacterium Combo Pack is perfect for scientists who require multiple agrobacterium competent cell strains for their research. The ElectroCompetent Agrobacterium Combo Pack contains 3 50µl aliquots of GV3101, AGL1, EHA105 and LBA4404 along with pCambia and our proprietary Agro Recovery Media. The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn. The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.