Biosensis is proud to offer the first commercially available ApoE/β-amyloid (ApoE/Aβ) complex ELISA kit. As a result of extensive collaboration with Dr. LaDu's laboratory at UIC and validation by Biosensis, this ELISA can be used to accurately and consistently measure the extent of ApoE/Aβ complex in tissue extracts and other samples. The Biosensis ApoE/Aβ Complex ELISA kit is a sandwich ELISA and consists of a pre-coated mouse monoclonal anti-Aβ capture antibody, a highly validated ApoE/Aβ complex standard that is pre-formed, lyophilized and ready for reconstitution, a biotinylated ApoE detection antibody, and horseradish peroxidase (HRP)-conjugated streptavidin and detection reagent. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the level of ApoE/Aβ complex present in samples and protein standards. Importantly, a well-characterized and unique ApoE/Aβ complex is included as a standard. This complex is pre-formed and lyophilized, requiring only reconstitution with assay diluent prior to use. In order to assess non-specific ApoE protein binding, each kit includes additional plates pre-coated with control antibody.

The purpose of this kit is the in vitro qualitative measurement of ApoE/Aβ complexes in brain extracts and CSF samples from both transgenic mice and humans or primates, relative to a known ApoE/Aβ complex standard, only if used as directed. This kit has not been tested for other sample applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.